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Responses of Detoxification Enzymes and Genes to Nicosulfuron Stress in Two Genotypes of Sweet Corn Differing in Nicosulfuron Tolerance

Received: 15 October 2019     Accepted: 12 November 2019     Published: 4 December 2019
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Abstract

Nicosulfuron, a sulfonylurea herbicide is registered for use on field, controns many annual and perennial grass weeds. The accumulation of nicosulfuron will increase toxicity and induce unfavorable oxidative stress in plants. Acetolactate synthase (ALS, EC 2.2.1.6) and glutathione transferases (GSTs, E. C.2.5.1.18), which participate in detoxification of xenobiotics and limit oxidative damages of cellular macromolecules, are important groups of cytoprotective enzymes. This study aims to investigate the toxic effect of nicosulfuron on the detoxification enzyme activities and gene expressions in maize seedling. Specifically, Nicosulfuron-tolerant sweet inbred line ‘JP233’ and nicosulfuron-sensitive sweet inbred line ‘Z2H4’ are subjected to 80mg kg–1 nicosulfuron treatment when the fourth leaves are fully developed, and the resulting effects are compared to those processed by water. After nicosulfuron treatment, it is found that ALS and GST enzymes activities of Z2H4 are significantly lower than those of JP233. Compared to Z2H4, nicosulfuron treatment increases the expression levels of GST1, ALS1 and ALS2 genes in JP233. These results suggest that the increased transcription level of these detoxifying enzymes might play a vital role in reducing the toxicity of nicosulfuron and the oxidative stress induced by nicosulfuron in maize seedlings. The research will improve our understanding of the function of maize detoxification enzymes and genes in herbicide metabolism.

Published in Journal of Plant Sciences (Volume 7, Issue 6)
DOI 10.11648/j.jps.20190706.15
Page(s) 158-163
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Nicosulfuron, Sweet Corn, Acetolactate Synthase, Glutathione Transferase, Gene Expression, Oxidative Stress, Toxicity

References
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  • APA Style

    Guihua Lv, Guojin Guo, Jianjian Chen, Xiangnan Li, Zhenxing Wu. (2019). Responses of Detoxification Enzymes and Genes to Nicosulfuron Stress in Two Genotypes of Sweet Corn Differing in Nicosulfuron Tolerance. Journal of Plant Sciences, 7(6), 158-163. https://doi.org/10.11648/j.jps.20190706.15

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    ACS Style

    Guihua Lv; Guojin Guo; Jianjian Chen; Xiangnan Li; Zhenxing Wu. Responses of Detoxification Enzymes and Genes to Nicosulfuron Stress in Two Genotypes of Sweet Corn Differing in Nicosulfuron Tolerance. J. Plant Sci. 2019, 7(6), 158-163. doi: 10.11648/j.jps.20190706.15

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    AMA Style

    Guihua Lv, Guojin Guo, Jianjian Chen, Xiangnan Li, Zhenxing Wu. Responses of Detoxification Enzymes and Genes to Nicosulfuron Stress in Two Genotypes of Sweet Corn Differing in Nicosulfuron Tolerance. J Plant Sci. 2019;7(6):158-163. doi: 10.11648/j.jps.20190706.15

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  • @article{10.11648/j.jps.20190706.15,
      author = {Guihua Lv and Guojin Guo and Jianjian Chen and Xiangnan Li and Zhenxing Wu},
      title = {Responses of Detoxification Enzymes and Genes to Nicosulfuron Stress in Two Genotypes of Sweet Corn Differing in Nicosulfuron Tolerance},
      journal = {Journal of Plant Sciences},
      volume = {7},
      number = {6},
      pages = {158-163},
      doi = {10.11648/j.jps.20190706.15},
      url = {https://doi.org/10.11648/j.jps.20190706.15},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20190706.15},
      abstract = {Nicosulfuron, a sulfonylurea herbicide is registered for use on field, controns many annual and perennial grass weeds. The accumulation of nicosulfuron will increase toxicity and induce unfavorable oxidative stress in plants. Acetolactate synthase (ALS, EC 2.2.1.6) and glutathione transferases (GSTs, E. C.2.5.1.18), which participate in detoxification of xenobiotics and limit oxidative damages of cellular macromolecules, are important groups of cytoprotective enzymes. This study aims to investigate the toxic effect of nicosulfuron on the detoxification enzyme activities and gene expressions in maize seedling. Specifically, Nicosulfuron-tolerant sweet inbred line ‘JP233’ and nicosulfuron-sensitive sweet inbred line ‘Z2H4’ are subjected to 80mg kg–1 nicosulfuron treatment when the fourth leaves are fully developed, and the resulting effects are compared to those processed by water. After nicosulfuron treatment, it is found that ALS and GST enzymes activities of Z2H4 are significantly lower than those of JP233. Compared to Z2H4, nicosulfuron treatment increases the expression levels of GST1, ALS1 and ALS2 genes in JP233. These results suggest that the increased transcription level of these detoxifying enzymes might play a vital role in reducing the toxicity of nicosulfuron and the oxidative stress induced by nicosulfuron in maize seedlings. The research will improve our understanding of the function of maize detoxification enzymes and genes in herbicide metabolism.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - Responses of Detoxification Enzymes and Genes to Nicosulfuron Stress in Two Genotypes of Sweet Corn Differing in Nicosulfuron Tolerance
    AU  - Guihua Lv
    AU  - Guojin Guo
    AU  - Jianjian Chen
    AU  - Xiangnan Li
    AU  - Zhenxing Wu
    Y1  - 2019/12/04
    PY  - 2019
    N1  - https://doi.org/10.11648/j.jps.20190706.15
    DO  - 10.11648/j.jps.20190706.15
    T2  - Journal of Plant Sciences
    JF  - Journal of Plant Sciences
    JO  - Journal of Plant Sciences
    SP  - 158
    EP  - 163
    PB  - Science Publishing Group
    SN  - 2331-0731
    UR  - https://doi.org/10.11648/j.jps.20190706.15
    AB  - Nicosulfuron, a sulfonylurea herbicide is registered for use on field, controns many annual and perennial grass weeds. The accumulation of nicosulfuron will increase toxicity and induce unfavorable oxidative stress in plants. Acetolactate synthase (ALS, EC 2.2.1.6) and glutathione transferases (GSTs, E. C.2.5.1.18), which participate in detoxification of xenobiotics and limit oxidative damages of cellular macromolecules, are important groups of cytoprotective enzymes. This study aims to investigate the toxic effect of nicosulfuron on the detoxification enzyme activities and gene expressions in maize seedling. Specifically, Nicosulfuron-tolerant sweet inbred line ‘JP233’ and nicosulfuron-sensitive sweet inbred line ‘Z2H4’ are subjected to 80mg kg–1 nicosulfuron treatment when the fourth leaves are fully developed, and the resulting effects are compared to those processed by water. After nicosulfuron treatment, it is found that ALS and GST enzymes activities of Z2H4 are significantly lower than those of JP233. Compared to Z2H4, nicosulfuron treatment increases the expression levels of GST1, ALS1 and ALS2 genes in JP233. These results suggest that the increased transcription level of these detoxifying enzymes might play a vital role in reducing the toxicity of nicosulfuron and the oxidative stress induced by nicosulfuron in maize seedlings. The research will improve our understanding of the function of maize detoxification enzymes and genes in herbicide metabolism.
    VL  - 7
    IS  - 6
    ER  - 

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Author Information
  • Dongyang Maize Research Institute of Zhejiang Province, Zhejiang Academy of Agricultural Sciences, Dongyang, P. R. China

  • Dongyang Maize Research Institute of Zhejiang Province, Zhejiang Academy of Agricultural Sciences, Dongyang, P. R. China

  • Dongyang Maize Research Institute of Zhejiang Province, Zhejiang Academy of Agricultural Sciences, Dongyang, P. R. China

  • Dongyang Maize Research Institute of Zhejiang Province, Zhejiang Academy of Agricultural Sciences, Dongyang, P. R. China

  • Dongyang Maize Research Institute of Zhejiang Province, Zhejiang Academy of Agricultural Sciences, Dongyang, P. R. China

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