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Optimizing Growth Regulators for Micropropagation of Industrially Adaptable Eucalyptus Hybrids

Received: 23 May 2024     Accepted: 11 June 2024     Published: 27 June 2024
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Abstract

Eucalyptus is one of the highly economic tree species in the developing countries like India. In the present investigation, experiments on optimizing plant growth regulators in the micropropagation of promising inter specific Eucalyptus hybrid clones namely TNPL 191(E. camaldulensis× E. teriticornis), TNPL 192 (E. camaldulensis × E. pellita) and intra specific hybrid clone TNPL 193 (E. camaldulensis× E. camaldulensis) were conducted, following standard protocols developed for Eucalyptus. The results showed that the BAP concentration of 0.50 mg L-1 for bud induction, IAA concentration of 3.0 mg L-1 for shoot elongation and IBA concentration of 1.0 mg L-1 for rooting of all these clones were found optimal. However, these three hybrid clones responded differently to the concentration of BAP at shoot proliferation stage. While the hybrid TNPL 191 showed maximum shoot proliferation rate at the concentration of 0.2 mg L-1 of BAP, the hybrids TNPL 192 and TNPL 193 showed highest response at 0.15 mg L-1. All the in vitro rooted plantlets were acclimatized successfully to the prevailing natural environment. Thus, the protocols developed with respect to optimizing the plant growth regulators can be adapted in large scale micro propagation of inter and intra specific Eucalyptus hybrid clones.

Published in Journal of Plant Sciences (Volume 12, Issue 3)
DOI 10.11648/j.jps.20241203.13
Page(s) 82-89
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Eucalyptus Hybrid, Micropropagation, BAP, IAA, IBA

References
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  • APA Style

    Naickar, M. C., Palanisamy, C., Vazram, P., Kuppusamy, J., Thangavel, S., et al. (2024). Optimizing Growth Regulators for Micropropagation of Industrially Adaptable Eucalyptus Hybrids. Journal of Plant Sciences, 12(3), 82-89. https://doi.org/10.11648/j.jps.20241203.13

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    ACS Style

    Naickar, M. C.; Palanisamy, C.; Vazram, P.; Kuppusamy, J.; Thangavel, S., et al. Optimizing Growth Regulators for Micropropagation of Industrially Adaptable Eucalyptus Hybrids. J. Plant Sci. 2024, 12(3), 82-89. doi: 10.11648/j.jps.20241203.13

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    AMA Style

    Naickar MC, Palanisamy C, Vazram P, Kuppusamy J, Thangavel S, et al. Optimizing Growth Regulators for Micropropagation of Industrially Adaptable Eucalyptus Hybrids. J Plant Sci. 2024;12(3):82-89. doi: 10.11648/j.jps.20241203.13

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  • @article{10.11648/j.jps.20241203.13,
      author = {Malaimuthu Chinnama Naickar and Chezhian Palanisamy and Prasath Vazram and Jayakumar Kuppusamy and Stalin Thangavel and Rajesh Ramasamy},
      title = {Optimizing Growth Regulators for Micropropagation of Industrially Adaptable Eucalyptus Hybrids
    },
      journal = {Journal of Plant Sciences},
      volume = {12},
      number = {3},
      pages = {82-89},
      doi = {10.11648/j.jps.20241203.13},
      url = {https://doi.org/10.11648/j.jps.20241203.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20241203.13},
      abstract = {Eucalyptus is one of the highly economic tree species in the developing countries like India. In the present investigation, experiments on optimizing plant growth regulators in the micropropagation of promising inter specific Eucalyptus hybrid clones namely TNPL 191(E. camaldulensis× E. teriticornis), TNPL 192 (E. camaldulensis × E. pellita) and intra specific hybrid clone TNPL 193 (E. camaldulensis× E. camaldulensis) were conducted, following standard protocols developed for Eucalyptus. The results showed that the BAP concentration of 0.50 mg L-1 for bud induction, IAA concentration of 3.0 mg L-1 for shoot elongation and IBA concentration of 1.0 mg L-1 for rooting of all these clones were found optimal. However, these three hybrid clones responded differently to the concentration of BAP at shoot proliferation stage. While the hybrid TNPL 191 showed maximum shoot proliferation rate at the concentration of 0.2 mg L-1 of BAP, the hybrids TNPL 192 and TNPL 193 showed highest response at 0.15 mg L-1. All the in vitro rooted plantlets were acclimatized successfully to the prevailing natural environment. Thus, the protocols developed with respect to optimizing the plant growth regulators can be adapted in large scale micro propagation of inter and intra specific Eucalyptus hybrid clones.
    },
     year = {2024}
    }
    

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  • TY  - JOUR
    T1  - Optimizing Growth Regulators for Micropropagation of Industrially Adaptable Eucalyptus Hybrids
    
    AU  - Malaimuthu Chinnama Naickar
    AU  - Chezhian Palanisamy
    AU  - Prasath Vazram
    AU  - Jayakumar Kuppusamy
    AU  - Stalin Thangavel
    AU  - Rajesh Ramasamy
    Y1  - 2024/06/27
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    N1  - https://doi.org/10.11648/j.jps.20241203.13
    DO  - 10.11648/j.jps.20241203.13
    T2  - Journal of Plant Sciences
    JF  - Journal of Plant Sciences
    JO  - Journal of Plant Sciences
    SP  - 82
    EP  - 89
    PB  - Science Publishing Group
    SN  - 2331-0731
    UR  - https://doi.org/10.11648/j.jps.20241203.13
    AB  - Eucalyptus is one of the highly economic tree species in the developing countries like India. In the present investigation, experiments on optimizing plant growth regulators in the micropropagation of promising inter specific Eucalyptus hybrid clones namely TNPL 191(E. camaldulensis× E. teriticornis), TNPL 192 (E. camaldulensis × E. pellita) and intra specific hybrid clone TNPL 193 (E. camaldulensis× E. camaldulensis) were conducted, following standard protocols developed for Eucalyptus. The results showed that the BAP concentration of 0.50 mg L-1 for bud induction, IAA concentration of 3.0 mg L-1 for shoot elongation and IBA concentration of 1.0 mg L-1 for rooting of all these clones were found optimal. However, these three hybrid clones responded differently to the concentration of BAP at shoot proliferation stage. While the hybrid TNPL 191 showed maximum shoot proliferation rate at the concentration of 0.2 mg L-1 of BAP, the hybrids TNPL 192 and TNPL 193 showed highest response at 0.15 mg L-1. All the in vitro rooted plantlets were acclimatized successfully to the prevailing natural environment. Thus, the protocols developed with respect to optimizing the plant growth regulators can be adapted in large scale micro propagation of inter and intra specific Eucalyptus hybrid clones.
    
    VL  - 12
    IS  - 3
    ER  - 

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